Sutherland J B, Crawford D L, Pometto A L
Can J Microbiol. 1983 Oct;29(10):1253-7. doi: 10.1139/m83-195.
Streptomyces setonii strain 75Vi2 was grown at 45 degrees C in liquid media containing yeast extract and trans-cinnamic acid, p-coumaric acid, ferulic acid, or vanillin. Gas chromatography, thin-layer chromatography, and mass spectrometry showed that cinnamic acid was catabolized via benzaldehyde, benzoic acid, and catechol; p-coumaric acid was catabolized via p-hydroxybenzaldehyde, p-hydroxybenzoic acid, and protocatechuic acid; ferulic acid was catabolized via vanillin, vanillic acid, and protocatechuic acid. When vanillin was used as the initial growth substrate, it was catabolized via vanillic acid, guaiacol, and catechol. The inducible ring-cleavage dioxygenases catechol 1,2-dioxygenase and protocatechuate 3,4-dioxygenase were detected with an oxygen electrode in cell-free extracts of cultures grown in media with aromatic growth substrates and yeast extract.
西托链霉菌菌株75Vi2在含有酵母提取物和反式肉桂酸、对香豆酸、阿魏酸或香草醛的液体培养基中于45℃培养。气相色谱法、薄层色谱法和质谱分析表明，肉桂酸通过苯甲醛、苯甲酸和儿茶酚进行分解代谢；对香豆酸通过对羟基苯甲醛、对羟基苯甲酸和原儿茶酸进行分解代谢；阿魏酸通过香草醛、香草酸和原儿茶酸进行分解代谢。当香草醛用作初始生长底物时，它通过香草酸、愈创木酚和儿茶酚进行分解代谢。在用芳香族生长底物和酵母提取物培养的无细胞提取物中，用氧电极检测到了可诱导的环裂解双加氧酶儿茶酚1,2 -双加氧酶和原儿茶酸3,4 -双加氧酶。
