Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
Appl Environ Microbiol. 1990 Jan;56(1):298-300. doi: 10.1128/aem.56.1.298-300.1990.
Radiocarbon incorporation from pyruvate and serine into monomethylmercury by Desulfovibrio desulfuricans was consistent with the proposal that the methyl group originates from C-3 of serine. Immunodiagnostic assays measured 4 to 35 mug of tetrahydrofolate and 58 to 161 ng of cobalamin or a closely related cobalt porphyrin per g of cell protein in D. desulfuricans. The light-reversible inhibition of mercury methylation by propyl iodide in D. desulfuricans indicates methyl transfer by a cobalt porphyrin.
脱硫弧菌将丙酮酸和丝氨酸中的放射性碳掺入单甲基汞的过程与甲基基团来源于丝氨酸的 C-3 的说法一致。免疫诊断检测表明，脱硫弧菌每克细胞蛋白中含有 4 至 35 微克四氢叶酸和 58 至 161 纳克钴胺素或与之密切相关的钴卟啉。碘化丙基对脱硫弧菌中汞甲基化的光可逆抑制表明，甲基的转移是通过钴卟啉进行的。
