质子自杀法直接选择丙酮丁醇梭菌发酵突变株。
Direct Selection of Clostridium acetobutylicum Fermentation Mutants by a Proton Suicide Method.
机构信息
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina.
出版信息
Appl Environ Microbiol. 1990 Feb;56(2):578-80. doi: 10.1128/aem.56.2.578-580.1990.
Abstract
Clostridium acetobutylicum ATCC 10132 mutants altered in acetic acid synthesis or in the shift to solventogenesis were directly selected by a proton suicide method after mutagenic treatment, by using bromide and bromate as selective agents. The mutants were characterized according to their solvent and acid production. On the selection plates they differed in colony phenotype from the parent strain.
摘要
梭菌属丙酮丁醇梭菌 ATCC 10132 的突变体，无论是在乙酸合成还是在转向溶剂生成方面发生改变，在经过诱变处理后，都可以通过质子自杀方法直接选择，使用溴化物和溴酸盐作为选择性试剂。根据溶剂和酸产量对突变体进行了表征。在选择平板上，它们的菌落表型与原始菌株不同。
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