质子自杀法：直接筛选糖转运和发酵缺陷型突变体的通用方法。
Proton suicide: general method for direct selection of sugar transport- and fermentation-defective mutants.
作者信息
Winkelman J W, Clark D P
出版信息
J Bacteriol. 1984 Nov;160(2):687-90. doi: 10.1128/jb.160.2.687-690.1984.
Abstract
We devised a positive selection procedure for bacterial mutants incapable of producing acid from sugars by fermentation. The method relied on the production of elemental bromine from a mixture of bromide and bromate under acidic conditions. When wild-type Escherichia coli cells were plated on media containing a fermentable sugar and an equimolar mixture of bromide and bromate, most of the cells were killed but a variety of mutants unable to produce acid from the sugar survived. Among these mutants were those defective in (i) sugar uptake, (ii) the glycolytic pathway, and (iii) the excretion. There were also novel mutants with some presumed regulatory defects affecting fermentation.
摘要
我们设计了一种针对无法通过发酵从糖类产生酸的细菌突变体的阳性选择程序。该方法依赖于在酸性条件下由溴化物和溴酸盐的混合物产生元素溴。当野生型大肠杆菌细胞接种在含有可发酵糖以及等摩尔溴化物和溴酸盐混合物的培养基上时，大多数细胞死亡，但各种无法从该糖产生酸的突变体存活了下来。这些突变体包括：（i）糖摄取缺陷型；（ii）糖酵解途径缺陷型；（iii）排泄缺陷型。还有一些推测具有影响发酵的调控缺陷的新型突变体。
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