天然束丝藻藻丝体和纯培养硫丝菌对 HCO(3) 的固定作用。

HCO(3) Fixation by Naturally Occurring Tufts and Pure Cultures of Thiothrix nivea.

机构信息

Department of Biological Sciences, Florida International University, Miami, Florida 33199.

出版信息

Appl Environ Microbiol. 1990 Mar;56(3):730-8. doi: 10.1128/aem.56.3.730-738.1990.

DOI:10.1128/aem.56.3.730-738.1990

PMID:16348147

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC183414/

Abstract

Naturally occurring tufts of the mixotroph Thiothrix nivea blanketed the East Everglades (Dade County, Fla.) Chekika artesian well and runoff areas. The rate of HCO(3) fixation by these Thiothrix tufts was determined to be 14.0 +/- 5.4 nmol of HCO(3) per min per mg of dry weight, which reflected a growth rate of 5.0%/h. The addition of 10 mM glucose, ribose, acetate, or pyruvate or 0.05% Casamino Acids (Difco Laboratories, Detroit, Mich.) did not appear to alter the HCO(3) fixation rate. Whereas 1 mM acetate or 10 mM lactate, ethanol, glycerol, alpha-ketoglutarate, succinate, fumarate, or citrate slightly stimulated HCO(3) fixation, 5 to 10 mM malate inhibited HCO(3) fixation by 90%. Pure Thiothrix cultures isolated from Chekika fixed HCO(3) at rates as high as 29.9 +/- 2.8 nmol of HCO(3) per min per mg of dry weight in the presence of growth medium. Malate did not have a suppressive effect but rather slightly stimulated in vivo HCO(3) fixation.

摘要

自然生长的混合营养体硫丝菌（Thiothrix nivea）覆盖了东大沼泽地（佛罗里达州戴德县）切基卡自流井和径流区域。这些硫丝菌束的 HCO3 固定率被确定为 14.0±5.4 nmol HCO3 每分钟每毫克干重，反映出 5.0%/h 的生长速率。添加 10 mM 葡萄糖、核糖、乙酸盐或丙酮酸盐或 0.05% 氨基酸（密歇根州底特律市 Difco 实验室）似乎不会改变 HCO3 固定率。然而，1 mM 乙酸盐或 10 mM 乳酸盐、乙醇、甘油、α-酮戊二酸、琥珀酸盐、延胡索酸盐或柠檬酸盐略微刺激了 HCO3 的固定，而 5 至 10 mM 富马酸盐则抑制了 HCO3 的固定，抑制率达 90%。从切基卡分离出的纯硫丝菌在生长培养基中固定 HCO3 的速率高达 29.9±2.8 nmol HCO3 每分钟每毫克干重。富马酸盐没有抑制作用，而是轻微地刺激了体内 HCO3 的固定。