van Peer Ron, Punte Helma L M, de Weger Letty A, Schippers Bob
Willie Commelin Scholten Phytopathological Laboratory, Department of Plant Pathology, State University of Utrecht, Javalaan 20, 3742 CP Baarn, and Department of Plant Molecular Biology, Leiden University, 2311 VJ Leiden, The Netherlands.
Appl Environ Microbiol. 1990 Aug;56(8):2462-2470. doi: 10.1128/aem.56.8.2462-2470.1990.
An extensive colonization of the endorhizosphere by fluorescent pseudomonads was observed in tomato plants grown on artificial substrates. These studies reveal that a significantly higher percentage of pseudomonads obtained from the endorhizosphere (30%) reduced plant growth than those obtained from the root surface (4%). Lipopolysaccharide patterns, cell envelope protein patterns, and other biochemical characteristics indicated that Pseudomonas isolates obtained from the endorhizosphere are distinct from Pseudomonas isolates obtained from the root surface. Isolates from the endorhizosphere especially were able to recolonize the endorhizosphere of both sterile and nonsterile tomato roots. The ability of the endorhizosphere isolates to colonize the endorhizosphere significantly correlated with their agglutination by tomato root agglutinin but did not correlate with chemotaxis to seed exudates of tomato. No correlation between colonization of the endorhizosphere and agglutination by root agglutinin could be demonstrated for the root surface isolates. We propose that agglutination of specific Pseudomonas strains by root agglutinin is of importance in the initial phase of adherence of bacteria to the root surface.
在人工基质上生长的番茄植株中，观察到荧光假单胞菌在内根际大量定殖。这些研究表明，从内根际分离得到的假单胞菌（30%）中，抑制植物生长的比例显著高于从根表面分离得到的假单胞菌（4%）。脂多糖模式、细胞包膜蛋白模式和其他生化特征表明，从内根际分离得到的假单胞菌菌株与从根表面分离得到的假单胞菌菌株不同。特别是从内根际分离得到的菌株能够在无菌和非无菌番茄根的内根际重新定殖。内根际分离菌株定殖内根际的能力与其被番茄根凝集素凝集显著相关，但与对番茄种子渗出物的趋化性无关。对于根表面分离菌株，无法证明内根际定殖与根凝集素凝集之间存在相关性。我们认为，根凝集素对特定假单胞菌菌株的凝集在细菌粘附到根表面的初始阶段具有重要意义。
