Frommel M I, Nowak J, Lazarovits G
Ministerio de Ganaderia, Agricultura y Pesca, Millan 4703, Montevideo, Uruguay.
Plant Physiol. 1991 Jul;96(3):928-36. doi: 10.1104/pp.96.3.928.
A plant growth-promoting rhizobacterium, designated Ps JN and isolated from onion roots, was identified as a nonfluorescent Pseudomonas sp. The percentage of similarity of Ps JN to P. gladioli (NCPPB 1891), P. cichorii (NCPPB 943), and P. viridiflava (NCPPB 635), as determined from 135 biochemical and physiological tests was 77, 70, and 66%, respectively. Ps JN persisted through successive generations of in vitro cultured potato plantlets, both as endophytic and epiphytic populations. In vitro inoculated potato (Solanum tuberosum) nodal explants produced plantlets with significant increases in root number (24-196%), root dry weight (44-201%), haulm dry weight (14-151%), and stem length (26-28%) as compared with noninoculated control plants. Bacterization also enhanced leaf hair formation (55-110%), secondary root branching, and total plant lignin content (43%). Other root colonizing bacteria or heat-killed cells of Ps JN had no significant effect on plant growth. Detached leaves from in vitro grown control plants, when exposed to 19 degrees C and 50% relative humidity, lost 55% of their moisture content in 2.5 hours. Moisture loss by leaves of in vitro grown, bacterized plants, as well as greenhouse-acclimated, bacterized plants, and control plants, was less than 20%. Changes in stomatal closure appear to account for this difference.
从洋葱根部分离出的一株促进植物生长的根际细菌，命名为Ps JN，被鉴定为非荧光假单胞菌属。通过135项生化和生理测试确定，Ps JN与唐菖蒲假单胞菌（NCPPB 1891）、菊苣假单胞菌（NCPPB 943）和绿黄假单胞菌（NCPPB 635）的相似性百分比分别为77%、70%和66%。Ps JN作为内生菌和附生菌群体，在连续几代的离体培养马铃薯苗中都能存活。与未接种的对照植株相比，离体接种马铃薯（Solanum tuberosum）节段外植体产生的幼苗在根数（增加24 - 196%）、根干重（增加44 - 201%）、地上部干重（增加14 - 151%）和茎长（增加26 - 28%）方面有显著增加。接种细菌还增强了叶毛形成（增加55 - 110%）、侧根分支和植株总木质素含量（增加43%）。其他定殖于根际的细菌或Ps JN的热灭活细胞对植物生长没有显著影响。离体培养的对照植株的离体叶片在19摄氏度和50%相对湿度下，2.5小时内水分损失了55%。离体培养的接种细菌的植株、温室驯化的接种细菌的植株以及对照植株的叶片水分损失均小于20%。气孔关闭的变化似乎是造成这种差异的原因。
