Nautiyal C S, Dion P
Département de Phytologie, Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Québec, Canada G1K 7P4.
Appl Environ Microbiol. 1990 Aug;56(8):2576-2579. doi: 10.1128/aem.56.8.2576-2579.1990.
Microorganisms utilizing an opine as the sole carbon source were recovered from crown gall tumors, soil, and surface-disinfected potato tubers. The effect of the opines octopine, nopaline, succinamopine, and mannopine as selective substrates was compared with that of the auxin indoleacetic acid. Selection on octopine and indoleacetic acid favored the fluorescent pseudomonads, whereas mannopine allowed the frequent recovery of agrobacteria. Coryneforms which utilized succinamopine or mannopine were detected in soil, but not in tumors. Fungi growing on succinamopine or mannopine and a mannopine-utilizing Pseudomonas putida were isolated from tumor and soil, respectively.
从冠瘿瘤、土壤和表面消毒的马铃薯块茎中分离出了以冠瘿碱作为唯一碳源的微生物。将章鱼碱、胭脂碱、琥珀酰章鱼碱和甘露碱作为选择性底物的效果与生长素吲哚乙酸的效果进行了比较。在章鱼碱和吲哚乙酸上进行选择有利于荧光假单胞菌生长，而甘露碱则使土壤杆菌得以频繁分离。在土壤中检测到利用琥珀酰章鱼碱或甘露碱的棒状杆菌，但在肿瘤中未检测到。分别从肿瘤和土壤中分离出了在琥珀酰章鱼碱或甘露碱上生长的真菌以及一株利用甘露碱的恶臭假单胞菌。
