Génétique et Amélioration des Plantes, Institut National de la Recherche Agronomique, 78000 Versailles, France.
Proc Natl Acad Sci U S A. 1980 May;77(5):2693-7. doi: 10.1073/pnas.77.5.2693.
Agrobacterium Ti (tumor-inducing) plasmids, the causative agents of crown gall disease, fall into four genetic groups based on the patterns of octopine and nopaline synthesis (by crown gall tumors) and catabolism (by Agrobacterium tumefaciens) for which they are responsible. Two classes of Ti plasmids induce tumors that synthesize neither octopine nor nopaline. The existence of these Ti plasmids challenged the view that opines such as octopine and nopaline play a central role in crown gall biology. We now report the occurrence of an opine in tumors induced by one of these classes of Ti plasmids, the "null-type" plasmids typified by pTi Bo542. The opine was purified by biological enrichment based on its utilization by bacteria containing pTi Bo542 but not by bacteria lacking a Ti plasmid. The mass spectrum and biological properties of this opine are identical to those of agropine, an opine recently discovered in octopine-type tumors. We propose that null-type Ti plasmids now be named for their signal opine, agropine-type Ti plasmids.
根癌农杆菌 Ti（肿瘤诱导）质粒是引起冠瘿瘤病的病原体，根据它们负责的 octopine 和 nopalin 合成（由冠瘿瘤合成）和分解代谢（由根癌农杆菌合成）模式，可分为四个遗传群。两类 Ti 质粒诱导既不合成 octopine 也不合成 nopalin 的肿瘤。这些 Ti 质粒的存在挑战了这样一种观点，即 opines 如 octopine 和 nopalin 在冠瘿生物学中起着核心作用。我们现在报告了由其中一类 Ti 质粒（以 pTi Bo542 为代表的“空型”质粒）诱导的肿瘤中存在 opine。该 opine 通过基于其被含 pTi Bo542 的细菌利用而不被缺乏 Ti 质粒的细菌利用的生物学富集进行纯化。该 opine 的质谱和生物学特性与最近在 octopine 型肿瘤中发现的 agro pine 相同。我们建议现在将空型 Ti 质粒命名为它们的信号 opine，agropine 型 Ti 质粒。
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