Soybean and Alfalfa Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Bldg. 011, HH-19, BARC-West, Beltsville, Maryland 20705, and Battelle, Columbus, Ohio 43201.
Appl Environ Microbiol. 1990 Sep;56(9):2736-41. doi: 10.1128/aem.56.9.2736-2741.1990.
The hemoprotein content of 17 strains of Bradyrhizobium japonicum bacteroids from field-grown plants and the corresponding strains of cultured cells was determined spectrally. The major terminal oxidases, cytochromes (cyt) aa(3) and o, were present in all strains of cultured cells. cyt aa(3) was present in significant amounts in bacteroids only in strains of DNA homology group II. cyt o appeared to be present in bacteroids of all strains, and the average level was the same as in cultured cells. cyt b and c in the membrane fractions were higher in bacteroids of all strains compared with cultured cells. cyt P-450 was present in both the membrane and soluble fractions of bacteroids of most strains. The total P-450 content varied sixfold among strains. A CO-reactive hemoprotein, P-422, was present in the soluble fraction of all strains of cultured cells. P-422 may be a hemoglobinlike protein, and it was present in significant amounts in bacteroids only in DNA homology group I strains.
测定了从田间生长的植物中分离出的 17 株野生型大豆根瘤菌类菌体和相应的培养细胞的血红素蛋白含量。所有培养细胞的主要末端氧化酶细胞色素(cyt)aa(3)和 o 都存在。仅在 DNA 同源群 II 的菌株中,类菌体中存在大量的 cyt aa(3)。cyt o 似乎存在于所有菌株的类菌体中,其平均水平与培养细胞相同。与培养细胞相比,所有菌株的类菌体中膜部分的 cyt b 和 cyt c 更高。P-450 细胞色素存在于大多数菌株的类菌体的膜和可溶性部分中。总 P-450 含量在菌株间变化了六倍。一种 CO 反应性血红素蛋白 P-422 存在于所有培养细胞株的可溶性部分。P-422 可能是一种血红蛋白样蛋白,仅在 DNA 同源群 I 菌株的类菌体中大量存在。