Department of Soil Science, The University of Reading, London Road, Reading RG1 5AQ, United Kingdom.
Appl Environ Microbiol. 1990 Dec;56(12):3629-33. doi: 10.1128/aem.56.12.3629-3633.1990.
Al was found to penetrate the cell envelopes of both sensitive and tolerant Rhizobium strains and bind to DNA in vivo. Despite causing a reduction in viability, Al stimulated DNA synthesis in the sensitive strain, which suggested that an excision repair mechanism was operating. The Al-stimulated DNA synthesis was reduced by the simultaneous addition of chloramphenicol. In contrast to the sensitive strain, DNA synthesis was unaffected by Al binding to DNA in the tolerant strain. It is proposed that Al enters the cell and binds to the DNA helix, increasing stabilization and preventing successful replication. Different repair mechanisms appear to operate in response to Al in tolerant and sensitive strains.
铝被发现可以穿透敏感和耐受根瘤菌株的细胞包膜,并在体内与 DNA 结合。尽管铝会降低菌株的生存能力,但它刺激了敏感菌株的 DNA 合成,这表明一种切除修复机制正在发挥作用。同时添加氯霉素可以减少铝刺激的 DNA 合成。与敏感菌株不同,铝与耐受菌株的 DNA 结合对 DNA 合成没有影响。据推测,铝进入细胞并与 DNA 螺旋结合,增加稳定性并防止成功复制。在耐受和敏感菌株中,似乎有不同的修复机制对铝做出反应。