College of Marine Studies, University of Delaware, Lewes, Delaware 19958.
Appl Environ Microbiol. 1990 Dec;56(12):3643-8. doi: 10.1128/aem.56.12.3643-3648.1990.
The relationships among surface energy, adsorbed organic matter, and attached bacterial growth were examined by measuring the degradation of adsorbed ribulose-1,5-bisphosphate carboxylase (a common algal protein) by attached bacteria (Pseudomonas strain S9). We found that surface energy (work of adhesion of water) determined the amount and availability of adsorbed protein and, consequently, the growth of attached bacteria. Percent degradation of adsorbed ribulose-1,5-bisphosphate carboxylase decreased with increasing hydrophobicity of the surface (decreasing work of adhesion). As a result, growth rates of attached bacteria were initially higher on hydrophilic glass than on hydrophobic polyethylene. However, during long (6-h) incubations, growth rates increased with surface hydrophobicity because of increasing amounts of adsorbed protein. Together with previous studies, these results suggest that the number of attached bacteria over time will be a complex function of surface energy. Whereas both protein adsorption and bacterial attachment decrease with increasing surface energy, availability of adsorbed protein and consequently initial bacterial growth rates increase with surface energy.
通过测量附着细菌（假单胞菌 S9 株）对吸附的核酮糖-1,5-二磷酸羧化酶（一种常见的藻类蛋白）的降解，研究了表面能、吸附有机物和附着细菌生长之间的关系。我们发现表面能（水的附着力功）决定了吸附蛋白的数量和可用性，从而决定了附着细菌的生长。吸附的核酮糖-1,5-二磷酸羧化酶的降解百分比随着表面疏水性（附着力功降低）的增加而降低。因此，附着细菌在亲水玻璃上的初始生长速度高于疏水聚乙烯。然而，在长时间（6 小时）孵育过程中，由于吸附蛋白的增加，生长速度随着表面疏水性的增加而增加。结合以前的研究结果，这些结果表明，随着时间的推移，附着细菌的数量将是表面能的复杂函数。虽然蛋白质吸附和细菌附着都随着表面能的增加而减少，但吸附蛋白的可用性以及初始细菌生长速率随着表面能的增加而增加。
