College of Marine Studies, University of Delaware, Lewes, Delaware 19958.
Appl Environ Microbiol. 1992 Apr;58(4):1374-5. doi: 10.1128/aem.58.4.1374-1375.1992.
Ribulose 1,5-bisphosphate carboxylase was radiolabelled by in vitro translation, resulting in uniformly labelled ribulose 1,5-bisphosphate carboxylase, and also by reductive methylation. We investigated the degradation of the two forms of radiolabelled protein by natural bacterial populations. Although total hydrolysis of uniformly labelled protein and methylated protein was nearly equal, percent assimilation, respiration, and release as low-molecular-weight material were different. Radioactivity from uniformly labelled protein was approximately equally assimilated into cells, respired as H(2)O, and released as low-molecular-weight material, but radioactivity from the methylated protein was nearly all released as low-molecular-weight material, and little was assimilated or respired.
核酮糖-1,5-二磷酸羧化酶通过体外翻译被放射性标记，从而产生均一标记的核酮糖-1,5-二磷酸羧化酶，也可以通过还原甲基化来标记。我们研究了两种形式的放射性标记蛋白在自然细菌群体中的降解情况。尽管均一标记蛋白和甲基化蛋白的完全水解几乎相等，但百分率的同化、呼吸和作为低分子量物质的释放则不同。均一标记蛋白的放射性大约等量地被细胞同化，作为 H2O 进行呼吸，并作为低分子量物质释放，但甲基化蛋白的放射性几乎全部作为低分子量物质释放，很少被同化或呼吸。
