温度对固氮菌结构基因表达的钼和钒依赖性调节。
Temperature-Dependent Regulation by Molybdenum and Vanadium of Expression of the Structural Genes Encoding Three Nitrogenases in Azotobacter vinelandii.
机构信息
Nitrogen Fixation Laboratory, AFRC Institute of Plant Science Research, University of Sussex, Brighton BN1 9RQ, United Kingdom.
出版信息
Appl Environ Microbiol. 1991 Feb;57(2):622-4. doi: 10.1128/aem.57.2.622-624.1991.
Abstract
Temperature affects the expression of the three different nitrogenases in Azotobacter vinelandii. Molybdenum repressed the vnfH and anfH operons relatively more at 30 degrees C than at 20 degrees C; at 14 degrees C molybdenum did not repress these genes at all. Similarly, V repressed the anf operon at 30 degrees C but not at 20 or 14 degrees C. Mo was poorly transported into cells grown at the lower temperatures. A. vinelandii thus has the potential to synthesize any of the three nitrogenases at 14 to 20 degrees C regardless of the presence of Mo or V.
摘要
温度会影响固氮菌中三种不同氮酶的表达。钼在 30°C 时对 vnfH 和 anfH 操纵子的抑制作用比对 20°C 时更强；在 14°C 时，钼根本不抑制这些基因。同样，V 在 30°C 时抑制 anf 操纵子，但在 20°C 或 14°C 时则不然。钼在低温下生长的细胞中的运输能力较差。因此，固氮菌无论是否存在钼或 V，都有可能在 14 到 20°C 之间合成三种氮酶中的任何一种。
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