褐球固氮菌的钼和钒固氮酶。低温有利于钒固氮酶进行N2还原。
Molybdenum and vanadium nitrogenases of Azotobacter chroococcum. Low temperature favours N2 reduction by vanadium nitrogenase.
作者信息
Miller R W, Eady R R
机构信息
Plant Research Centre, Agriculture Canada, Ottawa, Ontario.
出版信息
Biochem J. 1988 Dec 1;256(2):429-32. doi: 10.1042/bj2560429.
Abstract
A comparison of the effect of temperature on the reduction of N2 by purified molybdenum nitrogenase and vanadium nitrogenase of Azotobacter chroococcum showed differences in behaviour. As the assay temperature was lowered from 30 degrees C to 5 degrees C N2 remained an effective substrate for V nitrogenase, but not Mo nitrogenase, since the specific activity for N2 reduction by Mo nitrogenase decreased 10-fold more than that of V nitrogenase. Activity cross-reactions between nitrogenase components showed the enhanced low-temperature activity to be associated with the Fe protein of V nitrogenase. The lower activity of homologous Mo nitrogenase components, although dependent on the ratio of MoFe protein to Fe protein, did not equal that of V nitrogenase even under conditions of high electron flux obtained at a 12-fold molar excess of Fe protein.
摘要
对温度对纯化的钼固氮酶和褐球固氮菌钒固氮酶还原N₂的影响进行比较，结果显示出行为上的差异。随着测定温度从30℃降至5℃，N₂仍然是钒固氮酶的有效底物，但不是钼固氮酶的有效底物，因为钼固氮酶还原N₂的比活性下降幅度比钒固氮酶大10倍。固氮酶组分之间的活性交叉反应表明，低温活性的增强与钒固氮酶的铁蛋白有关。同源钼固氮酶组分的活性较低，尽管依赖于钼铁蛋白与铁蛋白的比例，但即使在铁蛋白摩尔过量12倍获得的高电子通量条件下，也不等于钒固氮酶的活性。
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本文引用的文献
1
Liquid chromatographic-fluorescence determination of ammonia from nitrogenase reactions: a 2-min assay.液相色谱-荧光法测定固氮酶反应中的氨：2 分钟分析法。
Appl Environ Microbiol. 1984 May;47(5):1027-30. doi: 10.1128/aem.47.5.1027-1030.1984.
2
Second gene (nifH*) coding for a nitrogenase iron protein in Azotobacter chroococcum is adjacent to a gene coding for a ferredoxin-like protein.编码褐球固氮菌中固氮酶铁蛋白的第二个基因（nifH*）与一个编码类铁氧化还原蛋白的基因相邻。
EMBO J. 1986 Jun;5(6):1159-63. doi: 10.1002/j.1460-2075.1986.tb04341.x.
3
Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii.棕色固氮菌中一种替代固氮系统的证据。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7342-6. doi: 10.1073/pnas.77.12.7342.
4
A nitrogen pressure of 50 atmospheres does not prevent evolution of hydrogen by nitrogenase.50个大气压的氮气压力并不能阻止固氮酶释放氢气。
Science. 1984 Jun 8;224(4653):1095-7. doi: 10.1126/science.6585956.
5
Nitrogenase of Klebsiella pneumoniae. Kinetics of the dissociation of oxidized iron protein from molybdenum-iron protein: identification of the rate-limiting step for substrate reduction.肺炎克雷伯菌的固氮酶。氧化态铁蛋白与钼铁蛋白解离的动力学：底物还原限速步骤的鉴定。
Biochem J. 1983 Nov 1;215(2):393-403. doi: 10.1042/bj2150393.
6
Expression of an alternative nitrogen fixation system in Azotobacter vinelandii.棕色固氮菌中一种替代固氮系统的表达。
J Bacteriol. 1982 Jun;150(3):1244-51. doi: 10.1128/jb.150.3.1244-1251.1982.
7
Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins.肺炎克雷伯菌的固氮酶。组分蛋白的纯化及性质
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8
Nitrogenase.固氮酶
Nature. 1974 Jun 28;249(460):805-10. doi: 10.1038/249805a0.
9
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10
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