Analytical Chemistry and Microbiology Departments, University College, Cork, Ireland.
Appl Environ Microbiol. 1992 Jan;58(1):353-8. doi: 10.1128/aem.58.1.353-358.1992.
Pseudomonas sp. strain F113 was isolated from the rhizosphere of sugar beets and shown to inhibit a range of plant pathogenic fungi by producing an antibioticlike compound. An antibiotic-negative mutant strain, F113G22, was generated by transposon mutagenesis. This mutant has lost the ability to inhibit both bacterial and fungal microorganisms on high-iron medium. The antibioticlike compound was subsequently identified as 2,4-diacetylphloroglucinol (DAPG), and a high-pressure liquid chromatographic assay was developed for to detect it quantitatively in growth culture media and soil. The growth temperature had a direct bearing on DAPG production by strain F113, with maximum production at 12 degrees C. The iron concentration, pH, and oxygen had no influence on DAPG production by strain F113 under the assay conditions used. However, a low ratio of culture volume to surface area available to the microbe in the growth container was critical for optimum DAPG production. Different types of carbon sources influenced DAPG production by strain F113 to various degrees. For example, sucrose, fructose, and mannitol promoted high yields of DAPG by strain F113, whereas glucose and sorbose resulted in very poor DAPG production.
从甜菜根际土壤中分离到一株假单胞菌（Pseudomonas sp. strain F113），该菌能够产生一种类似抗生素的化合物，从而抑制多种植物病原真菌。通过转座子诱变，得到一株抗生素阴性突变株 F113G22。该突变株丧失了在高铁培养基上抑制细菌和真菌生长的能力。随后鉴定出这种类似抗生素的化合物为 2,4-二乙酰基间苯三酚（DAPG），并建立了一种高压液相色谱检测方法，用于定量检测生长培养基和土壤中的 DAPG。F113 菌株的生长温度对 DAPG 的产生有直接影响，最适生长温度为 12°C。在实验条件下，铁浓度、pH 值和氧气对 F113 菌株的 DAPG 产量没有影响。然而，生长容器中微生物可利用的培养体积与表面积的低比例对 DAPG 的最佳产量至关重要。不同类型的碳源对 F113 菌株的 DAPG 产量有不同程度的影响。例如，蔗糖、果糖和甘露醇促进 F113 菌株产生高产量的 DAPG，而葡萄糖和山梨醇则导致 DAPG 的产量非常低。
