Departments of Microbiology and Materials Science and Engineering and Advanced Materials Technology Center, University of Washington, Seattle, Washington 98195.
Appl Environ Microbiol. 1992 Sep;58(9):3130-5. doi: 10.1128/aem.58.9.3130-3135.1992.
The high surface charge of small ceramic particles such as alumina particles prevents them from dispersing evenly in aqueous suspensions and forming high-density compacts. However, suspensions of 400-nm-diameter alumina particles treated with alginate from the bacterium Azotobacter vinelandii were well dispersed. The alginate bound firmly to the particle surface and could not be removed by repeated washing with distilled water (2.82 mg of the bacterial alginate adsorbed to 1 g of the alumina particles). Furthermore, A. vinelandii grew and produced alginate in the presence of up to 15% (vol/vol) alumina particles. These results suggest that an in situ process using this bacterium to coat ceramic particles with alginate might be developed. In in situ processing experiments, the particle-packing densities were significantly increased and the viscosities of 5 and 10% (vol/vol) suspensions were reduced 4- and 60-fold, respectively, over those of controls. The bacteria were readily removed from the alumina particles by washing.
小陶瓷颗粒(如氧化铝颗粒)具有较高的表面电荷,这使得它们无法均匀分散在水悬浮液中,从而无法形成高密度的压坯。然而,经过来自土壤杆菌(Azotobacter vinelandii)的褐藻酸钠处理的 400nm 直径的氧化铝颗粒悬浮液则具有良好的分散性。褐藻酸钠牢固地结合在颗粒表面上,并且用蒸馏水反复洗涤(2.82mg 细菌褐藻酸钠吸附到 1g 氧化铝颗粒上)也无法去除。此外,在高达 15%(体积/体积)的氧化铝颗粒存在的情况下,土壤杆菌仍能生长并产生褐藻酸钠。这些结果表明,可能开发出一种使用该细菌原位处理来在陶瓷颗粒上涂覆褐藻酸钠的方法。在原位处理实验中,颗粒堆积密度显著增加,5%和 10%(体积/体积)悬浮液的粘度分别比对照降低了 4 倍和 60 倍。细菌很容易通过洗涤从氧化铝颗粒中去除。