Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géomatique, Université Laval, Sainte-Foy, Québec, Canada G1K 7P4, and Biotechnology Research Council, National Research Council of Canada, Montreal, Québec, Canada H4P 2R2.
Appl Environ Microbiol. 1992 Oct;58(10):3217-24. doi: 10.1128/aem.58.10.3217-3224.1992.
The white rot fungi Lentinula edodes, Phanerochaete chrysosporium, Pleurotus sajor-caju, Flammulina velutipes, and Schizophyllum commune were grown in liquid media containing C-lignin-labelled wood, and the formation of water-soluble C-labelled products and CO(2), the growth of the fungi, and the activities of extracellular lignin peroxidase, manganese peroxidase, and laccase were measured. Conditions that affect the rate of lignin degradation were imposed, and both long-term (0- to 16-day) and short-term (0- to 72-h) effects on the production of the two types of product and on the activities of the enzymes were monitored. The production of CO(2)-labelled products from the aqueous ones was also investigated. The short-term studies showed that the different conditions had different effects on the production of the two products and on the activities of the enzymes. Nitrogen sources inhibited the production of both products by all species when differences in growth could be discounted. Medium pH and manganese affected lignin degradation by the different species differently. With P. chrysosporium, the results were consistent, with lignin peroxidase playing a role in lignin solubilization and manganese peroxidase being important in subsequent CO(2) production.
白腐真菌香菇、糙皮侧耳、姬松茸、金针菇和裂褶菌在含有 C 标记木质素的液体培养基中生长，测定了水溶性 C 标记产物和 CO2 的形成、真菌的生长以及胞外木质素过氧化物酶、锰过氧化物酶和漆酶的活性。施加了影响木质素降解速率的条件，并监测了两种产物的产生以及酶活性的长期（0 至 16 天）和短期（0 至 72 小时）影响。还研究了从水溶液中产生的 CO2 标记产物。短期研究表明，不同条件对两种产物的产生以及酶活性有不同的影响。氮源抑制了所有物种两种产物的产生，当可以忽略生长差异时。培养基 pH 值和锰对不同物种的木质素降解有不同的影响。对于 P. chrysosporium，结果是一致的，木质素过氧化物酶在木质素溶解中起作用，而锰过氧化物酶在随后的 CO2 产生中很重要。
