Périé F H, Gold M H
Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.
Appl Environ Microbiol. 1991 Aug;57(8):2240-5. doi: 10.1128/aem.57.8.2240-2245.1991.
Extracellular manganese peroxidase and laccase activities were detected in cultures of Dichomitus squalens (Polyporus anceps) under conditions favoring lignin degradation. In contrast, neither extracellular lignin peroxidase nor aryl alcohol oxidase activity was detected in cultures grown under a wide variety of conditions. The mineralization of 14C-ring-, -side chain-, and -methoxy-labeled synthetic guaiacyl lignins by D. squalens and the expression of extracellular manganese peroxidase were dependent on the presence of Mn(II), suggesting that manganese peroxidase is an important component of this organism's lignin degradation system. The expression of laccase activity was independent of manganese. In contrast to previous findings with Phanerochaete chrysosporium, lignin degradation by D. squalens proceeded in the cultures containing excess carbon and nitrogen.
在有利于木质素降解的条件下，检测到了斜生纤孔菌（多孔菌属）培养物中的细胞外锰过氧化物酶和漆酶活性。相比之下，在多种条件下培养的培养物中均未检测到细胞外木质素过氧化物酶和芳醇氧化酶活性。斜生纤孔菌对14C环、侧链和甲氧基标记的合成愈创木基木质素的矿化作用以及细胞外锰过氧化物酶的表达取决于Mn(II)的存在，这表明锰过氧化物酶是该生物体木质素降解系统的重要组成部分。漆酶活性的表达与锰无关。与之前对黄孢原毛平革菌的研究结果相反，斜生纤孔菌在含有过量碳和氮的培养物中进行木质素降解。
