内源性储存脂质聚-β-羟基丁酸对甲烷营养混合培养物共代谢三氯乙烯和萘时还原力可用性的影响。
Influence of the Endogenous Storage Lipid Poly-beta-Hydroxybutyrate on the Reducing Power Availability during Cometabolism of Trichloroethylene and Naphthalene by Resting Methanotrophic Mixed Cultures.
机构信息
Environmental Engineering and Science, Department of Civil Engineering, Stanford University, Stanford, California 94305-4020.
出版信息
Appl Environ Microbiol. 1993 May;59(5):1602-6. doi: 10.1128/aem.59.5.1602-1606.1993.
Abstract
The role of the storage lipid poly-beta-hydroxybutyrate (PHB) in trichloroethylene transformation by methanotrophic mixed cultures was investigated. Naphthalene oxidation rates were used to assay for soluble methane monooxygenase activity. The PHB content of methanotrophic cells grown in reactors varied diurnally as well as from day to day. A positive correlation between the amount of PHB in the cells and the naphthalene oxidation rate as well as between PHB and the trichloroethylene transformation rate and capacity was found. Addition of beta-hydroxybutyrate increased the naphthalene oxidation rates significantly. PHB content in cells could be manipulated by incubation at different methane-to-nitrogen ratios. A positive correlation between the naphthalene oxidation rate and the PHB content after these incubations could be seen. Both the PHB content and the naphthalene oxidation rates decreased with time in resting methanotrophic cells exposed to oxygen. However, this decrease in the naphthalene oxidation rate cannot be explained by the decrease in the PHB content alone. Probably a deactivation of the methane monooxygenase itself is also involved.
摘要
研究了储存脂质多-羟基丁酸酯 (PHB) 在甲烷营养混合培养物转化三氯乙烯中的作用。萘氧化速率用于测定可溶性甲烷单加氧酶活性。在反应器中生长的甲烷营养细胞的 PHB 含量随昼夜和每天的变化而变化。发现细胞中 PHB 的含量与萘的氧化速率以及 PHB 与三氯乙烯转化速率和容量之间存在正相关关系。添加β-羟基丁酸显著增加了萘的氧化速率。通过在不同的甲烷与氮比下孵育可以操纵细胞中的 PHB 含量。可以看到这些孵育后萘氧化速率与 PHB 含量之间存在正相关关系。在有氧暴露下处于静止状态的甲烷营养细胞中，PHB 含量和萘氧化速率随时间的推移而降低。然而，这种萘氧化速率的降低不能仅用 PHB 含量的降低来解释。可能甲烷单加氧酶本身的失活也参与其中。
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