Key Laboratory of Industrial Ecology and Environmental Engineering, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Key Laboratory of Industrial Ecology and Environmental Engineering, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
J Environ Sci (China). 2018 Mar;65:133-143. doi: 10.1016/j.jes.2017.03.016. Epub 2017 Mar 28.
Methanotrophic-heterotrophic communities were selectively enriched from sewage sludge to obtain a mixed culture with high levels of poly-β-hydroxybutyrate (PHB) accumulation capacity from methane. Methane was used as the carbon source, N as sole nitrogen source, and oxygen and Cu content were varied. Copper proved essential for PHB synthesis. All cultures enriched with Cu could accumulate high content of PHB (43.2%-45.9%), while only small amounts of PHB were accumulated by cultures enriched without Cu (11.9%-17.5%). Batch assays revealed that communities grown with Cu and a higher O content synthesized more PHB, which had a wider optimal CH:O range and produced a high PHB content (48.7%) even though in the presence of N. In all methanotrophic-heterotrophic communities, both methanotrophic and heterotrophic populations showed the ability to accumulate PHB. Although methane was added as the sole carbon source, heterotrophs dominated with abundances between 77.2% and 85.6%. All methanotrophs detected belonged to type II genera, which formed stable communities with heterotrophs of different PHB production capacities.
从污水污泥中选择性富集甲烷营养型异养群落，以获得一种具有高聚β-羟基丁酸（PHB）积累能力的混合培养物。以甲烷为碳源，N 为唯一氮源，改变氧和 Cu 含量。铜被证明是 PHB 合成的必需元素。所有用 Cu 富集的培养物都能积累高含量的 PHB（43.2%-45.9%），而没有 Cu 富集的培养物只能积累少量的 PHB（11.9%-17.5%）。分批实验表明，在 Cu 和较高 O 含量下生长的群落合成更多的 PHB，具有更宽的最佳 CH：O 范围，即使在 N 的存在下也能产生高 PHB 含量（48.7%）。在所有的甲烷营养型异养群落中，甲烷营养型和异养型种群都表现出积累 PHB 的能力。尽管甲烷被添加为唯一的碳源，但异养菌的丰度在 77.2%至 85.6%之间占主导地位。所有检测到的甲烷营养菌都属于 II 型属，与不同 PHB 生产能力的异养菌形成稳定的群落。
