Laboratory of Microbial Gene Technology, The Norwegian Research Council, P.O. Box 5051, N-1432 As, Norway.
Appl Environ Microbiol. 1993 Jun;59(6):1966-8. doi: 10.1128/aem.59.6.1966-1968.1993.
Plasmids carrying the cohesive end region from temperate lactococcal bacteriophage PhiLC3 could be packaged in vivo by PhiLC3 and transduced into its host strain, Lactococcus lactis subsp. cremoris NCDO 1201. The transduction frequencies were between 10 and 10 transducing particles per PFU, depending on the size of the phage DNA insert. This transduction system is limited to only certain lactococcal strains. The PhiLC3 cohesive site region (cos) appears to play an important role in plasmid transduction.
携带温和乳球菌噬菌体 PhiLC3 粘性末端区域的质粒可被 PhiLC3 包装到体内，并转导到其宿主菌株乳酸乳球菌亚种。cremoris NCDO 1201 中。转导频率在每个 PFU 之间为 10 到 10 个转导颗粒，具体取决于噬菌体 DNA 插入片段的大小。该转导系统仅限于某些乳球菌菌株。PhiLC3 粘性位点区域（cos）似乎在质粒转导中起着重要作用。
