van de Guchte M, Daly C, Fitzgerald G F, Arendt E K
National Food Biotechnology Centre, University College, Cork, Ireland.
Appl Environ Microbiol. 1994 Jul;60(7):2324-9. doi: 10.1128/aem.60.7.2324-2329.1994.
The DNA sequence of the int-attP region of the small-isometric-headed lactococcal bacteriophage Tuc2009 is presented. In this region, an open reading frame, int, which potentially encodes a protein of 374 amino acids, representing the Tuc2009 integrase, was identified. The nucleotide sequence of the bacteriophage attachment site, attP, and the sequences of attB, attL, and attR in the lysogenic host Lactococcus lactis subsp. cremoris UC509 were determined. A sequence almost identical to the UC509 attB sequence was found to be present in the plasmid-free Tuc2009-resistant L. lactis subsp. cremoris MG1363. This site could be used for the site-specific integration of a plasmid carrying the Tuc2009 int-attP region in the chromosome of MG1363, thereby demonstrating that the application of chromosomal insertion vectors based on bacteriophage integration functions is not limited to the prophage-cured original host strain of the phage.
本文展示了小等轴头乳酸球菌噬菌体Tuc2009的int-attP区域的DNA序列。在该区域，鉴定出一个开放阅读框int，它可能编码一个由374个氨基酸组成的蛋白质，即Tuc2009整合酶。测定了噬菌体附着位点attP的核苷酸序列以及溶源性宿主乳酸乳球菌亚种cremoris UC509中的attB、attL和attR序列。在无质粒的抗Tuc2009乳酸乳球菌亚种cremoris MG1363中发现了一个与UC509 attB序列几乎相同的位点。该位点可用于将携带Tuc2009 int-attP区域的质粒位点特异性整合到MG1363的染色体中，从而证明基于噬菌体整合功能的染色体插入载体的应用并不局限于噬菌体的原噬菌体治愈原始宿主菌株。
