从土壤中用聚合酶链反应扩增 DNA 的聚乙烯吡咯烷酮-琼脂糖凝胶电泳纯化。
Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils.
机构信息
Exxon Research and Engineering Company, Route 22 East, Annandale, New Jersey 08801.
出版信息
Appl Environ Microbiol. 1993 Jun;59(6):1972-4. doi: 10.1128/aem.59.6.1972-1974.1993.
Abstract
This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards the electrophoretic mobility of denaturing phenolic compounds so that they do not comigrate with nucleic acids.
摘要
本通讯描述了琼脂糖凝胶电泳的一种改良方法，可从土壤中快速简单地纯化聚合酶链反应扩增的 DNA。这种改良方法是在琼脂糖凝胶中添加聚乙烯吡咯烷酮。聚乙烯吡咯烷酮的添加会降低变性酚类化合物的电泳迁移率，从而使其不会与核酸共迁移。
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