National Research Institute of Vegetables, Ornamental Plants and Tea, 2769 Kanaya, Shizuoka 428, and Kyushu National Agricultural Experiment Station, Suya, Nishigoshi, Kumamoto 861-11, Japan.
Appl Environ Microbiol. 1993 Jul;59(7):2121-5. doi: 10.1128/aem.59.7.2121-2125.1993.
A bacterium capable of hydrolyzing carbaryl (1-naphthyl-N-methylcarbamate) was isolated from a soil enrichment. This bacterium was characterized taxonomically as a Blastobacter sp. and designated strain M501. A carbaryl hydrolase present in this strain was purified to homogeneity by protamine sulfate treatment, ammonium sulfate precipitation, and hydrophobic, anion-exchange, gel filtration, and hydroxylapatite chromatographies. The native enzyme had a molecular mass of 166,000 Da and was composed of two subunits with molecular masses of 84,000 Da. The optimum pH and temperature of the enzyme activity were 9.0 and 45 degrees C, respectively. The enzyme was not stable at temperatures above 40 degrees C. The purified enzyme hydrolyzed seven N-methylcarbamate insecticides and also exhibited activity against 1-naphthyl acetate and 4-nitrophenyl acetate.
从土壤中分离到可水解甲萘威（1-萘基-N-甲基氨基甲酸酯）的细菌。该细菌在分类学上被鉴定为 Blastobacter sp.，并被命名为 M501 菌株。该菌株中存在的甲萘威水解酶通过鱼精蛋白硫酸盐处理、硫酸铵沉淀、疏水、阴离子交换、凝胶过滤和羟基磷灰石层析进行了纯化为均相。天然酶的分子量为 166,000 Da，由两个分子量为 84,000 Da 的亚基组成。该酶的最适 pH 和温度分别为 9.0 和 45 摄氏度。该酶在 40 摄氏度以上的温度下不稳定。纯化的酶可水解 7 种 N-甲基氨基甲酸酯类杀虫剂，也对 1-萘基乙酸酯和 4-硝基苯乙酸酯表现出活性。
