Hayatsu M, Hirano M, Tokuda S
Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Appl Environ Microbiol. 2000 Apr;66(4):1737-40. doi: 10.1128/AEM.66.4.1737-1740.2000.
A bacterium capable of utilizing fenitrothion (O,O-dimethyl O-4-nitro-m-tolyl phosphorothioate) as a sole carbon source was isolated from fenitrothion-treated soil. This bacterium was characterized taxonomically as being a member of the genus Burkholderia and was designated strain NF100. NF100 first hydrolyzed an organophosphate bond of fenitrothion, forming 3-methyl-4-nitrophenol, which was further metabolized to methylhydroquinone. The ability to degrade fenitrothion was found to be encoded on two plasmids, pNF1 and pNF2.
从经杀螟硫磷处理的土壤中分离出一种能够将杀螟硫磷(O,O-二甲基-O-4-硝基间甲苯基硫代磷酸酯)作为唯一碳源利用的细菌。经分类鉴定,该细菌属于伯克霍尔德菌属,被命名为菌株NF100。NF100首先水解杀螟硫磷的有机磷酸键,形成3-甲基-4-硝基苯酚,该物质进一步代谢为甲基对苯二酚。发现降解杀螟硫磷的能力由两个质粒pNF1和pNF2编码。