Center for Interfacial Microbial Process Engineering, Montana State University, Bozeman, Montana 59717.
Appl Environ Microbiol. 1993 Oct;59(10):3455-62. doi: 10.1128/aem.59.10.3455-3462.1993.
Three strains of Pseudomonas fluorescens with different motility rates and adsorption rate coefficients were injected into porous-medium reactors packed with l-mm-diameter glass spheres. Cell breakthrough, time to peak concentration, tailing, and cell recovery were measured at three interstitial pore velocities (higher than, lower than, and much lower than the maximal bacterial motility rate). All experiments were done with distilled water to reduce the effects of growth and chemotaxis. Contrary to expectations, motility did not result in either early breakthrough or early time to peak concentration at flow velocities below the motility rate. Bacterial size exclusion effects were shown to affect breakthrough curve shape at the very low flow velocity, but no such effect was seen at the higher flow velocity. The tendency of bacteria to adsorb to porous-medium surfaces, as measured by adsorption rate coefficients, profoundly influenced transport characteristics. Cell recoveries were shown to be correlated with the ratio of advective to adsorptive transport in the reactors. Adsorption rate coefficients were found to be better predictors of microbial transport phenomena than individual characteristics, such as size, motility, or porous-medium hydrodynamics.
三种不同运动速率和吸附速率系数的荧光假单胞菌菌株被注入填充有 1 毫米直径玻璃珠的多孔介质反应器中。在三个孔隙速度（高于、低于和远低于最大细菌运动速率）下测量细胞突破、达到峰值浓度的时间、尾部和细胞回收。所有实验均使用蒸馏水进行，以减少生长和趋化性的影响。与预期相反，在流速低于运动速率的情况下，运动并没有导致早期突破或早期达到峰值浓度。细菌大小排除效应在非常低的流速下影响突破曲线形状，但在较高的流速下没有观察到这种效应。细菌对多孔介质表面的吸附倾向，如吸附速率系数所测量的，深刻地影响了传输特性。细胞回收率与反应器中推流和吸附传输的比值相关。吸附速率系数被发现比单个特征（如大小、运动性或多孔介质流体动力学）更好地预测微生物传输现象。
