Sutar Vinayak P, Singh Varsha K, Sinha Rajeshwar P
Department of Microbiology, Deshbhakt Anandrao Balwantrao Naik Arts and Science College, Chikhali, Sangli, 415408, Maharashtra, India.
Laboratory of Photobiology and Molecular Microbiology, Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
Braz J Microbiol. 2025 Mar;56(1):1-10. doi: 10.1007/s42770-024-01577-7. Epub 2024 Dec 4.
The disposal of fat, oil, and grease (FOG) pollutants from various sources, including restaurants, food processing facilities, and domestic kitchens, poses significant challenges to wastewater treatment systems. In this study, we isolated and characterized a novel bacterial strain. The result of 16 S rRNA gene and phylogenetic analysis showed that the isolate was Staphylococcus petrasii sub sp. jettensis and named as VSJK R1 (Fig. S1). It was tested for its biodegradation potential of FOG contaminants. Our investigation revealed that Staphylococcus petrasii sub sp. jettensis VSJK R1 effectively degraded a variety of edible oils, including soybean, sunflower, cottonseed, palm, groundnut, and butter, with notable efficiency. Optimization studies were conducted to determine the optimal conditions for biodegradation, including the effects of nitrogen, carbon, phosphorus, pH, temperature, and salt concentration. Results indicated that organic nitrogen sources and glucose as carbon source significantly enhanced biodegradation rates, while the addition of phosphorus further improved degradation efficiency within specific concentration ranges. Moreover, the optimal pH for biodegradation was found to be neutral, with temperature ranging between 22°C and 45°C favoring microbial activity. Remarkably, Staphylococcus petrasii sub sp. jettensis VSJK R1 exhibited resilience to high salt concentrations, making it suitable for treatment of wastewater with elevated salt content. Additionally, comparative studies with other microbial strains underscored the unique biodegradation capabilities of Staphylococcus petrasii sub sp. jettensis VSJK R1, particularly in degrading various edible oils. The results suggest promising applications of this novel isolate in bioremediation efforts targeting FOG pollutants in wastewater treatment plants and grease traps. Future research may focus on scaling up the bioremediation process and field testing the efficacy of Staphylococcus petrasii sub sp. jettensis VSJK R1 in real-world wastewater treatment scenarios.
来自包括餐馆、食品加工设施和家庭厨房在内的各种来源的脂肪、油和油脂（FOG）污染物的处理，给废水处理系统带来了重大挑战。在本研究中，我们分离并鉴定了一种新型细菌菌株。16S rRNA基因和系统发育分析结果表明，该分离株为佩氏葡萄球菌杰氏亚种，并命名为VSJK R1（图S1）。对其降解FOG污染物的潜力进行了测试。我们的调查显示，佩氏葡萄球菌杰氏亚种VSJK R1能有效降解多种食用油，包括大豆油、葵花籽油、棉籽油、棕榈油、花生油和黄油，效率显著。进行了优化研究以确定生物降解的最佳条件，包括氮、碳、磷、pH值、温度和盐浓度的影响。结果表明，有机氮源和葡萄糖作为碳源显著提高了生物降解率，而在特定浓度范围内添加磷进一步提高了降解效率。此外，发现生物降解的最佳pH值为中性，温度在22°C至45°C之间有利于微生物活性。值得注意的是，佩氏葡萄球菌杰氏亚种VSJK R1对高盐浓度具有耐受性，使其适用于处理含盐量较高的废水。此外，与其他微生物菌株的比较研究强调了佩氏葡萄球菌杰氏亚种VSJK R1独特的生物降解能力，特别是在降解各种食用油方面。结果表明，这种新型分离株在针对废水处理厂和油脂截留器中的FOG污染物的生物修复工作中具有广阔的应用前景。未来的研究可能集中在扩大生物修复过程的规模以及在实际废水处理场景中对佩氏葡萄球菌杰氏亚种VSJK R1的功效进行现场测试。
