培养基组成对脱氮副球菌硝酸盐反硝化的影响。
Effect of Medium Composition on the Denitrification of Nitrate by Paracoccus denitrificans.
机构信息
Institute of Microbiology, Warsaw University, Karowa 18, 00-324 Warsaw, Poland.
出版信息
Appl Environ Microbiol. 1993 Nov;59(11):3951-3. doi: 10.1128/aem.59.11.3951-3953.1993.
The course of denitrification of nitrate in static cultures of Paracoccus denitrificans was studied. Reduction of nitrate to gaseous nitrogen without accumulation of nitrite because of parallel and balanced activities of nitrate and nitrite reductases was observed in nutrient broth. In minimal liquid cultures supplemented with either methanol, acetate, or ethanol as a sole carbon source, substantial amounts of nitrite (up to 70%) accumulated. The reduction in nitrite concentration began just after the transformation of nitrate to nitrite was completed. The addition of some growth factors to minimal media shortened the bacterial biomass doubling time. A correlation coefficient of 0.71 between the doubling time and the amount of accumulated nitrite in cultures was found. My results indicated that the type of denitrification carried out by P. denitrificans is not stable and depends on the nutritional composition of the culture medium.
研究了副球菌属（Paracoccus denitrificans）在静态培养物中硝酸盐的脱硝过程。在营养肉汤中观察到由于硝酸盐和亚硝酸盐还原酶的平行和平衡活性，没有积累亚硝酸盐而将硝酸盐还原为气态氮。在补充甲醇、乙酸盐或乙醇作为唯一碳源的最小液体培养基中，大量的亚硝酸盐（高达 70%）积累。在硝酸盐完全转化为亚硝酸盐后，亚硝酸盐浓度的降低才开始。向最小培养基中添加一些生长因子会缩短细菌生物量的倍增时间。在培养物中，倍增时间和积累的亚硝酸盐量之间的相关系数为 0.71。我的结果表明，副球菌属进行的脱硝类型不稳定，取决于培养基的营养成分。
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