Xiujie Wang, Weiqi Wang, Jing Zhang, Siyu Wang, Jun Li
The College of Architecture and Civil Engineering, Bejing University of Technology Beijing 100124 China
RSC Adv. 2019 Apr 10;9(20):11263-11271. doi: 10.1039/c8ra09518c. eCollection 2019 Apr 9.
Partial denitrification (NO -N → NO -N) was combined with anaerobic ammonium oxidation (ANAMMOX) to achieve nitrogen removal with a low C/N ratio and low energy consumption. Three different acclimation conditions, namely, R1 (sequencing batch reactor (SBR) under anoxic conditions), R2 (SBR under alternating anoxic/aerobic conditions), and R3 (SBR under low-intensity aeration), were investigated using glucose as an electron donor to achieve continuous accumulation of nitrite during a 120 d run. Subsequently, the denitrification performance and microbial community structure of the sludge were investigated. The results showed that the acclimatized sludge in reactors R2 and R3 achieved better partial denitrification performance than the sludge in R1 due to the presence of dissolved oxygen as a result of aeration. Notably, the R3 reactor had the optimal conditions for nitrite accumulation. The high-throughput sequencing analysis indicated that the dominant bacteria in R2 and R3 were with a relative abundance of 45.44% and 34.96%, respectively. This was the first time that was reported as the dominant bacteria in denitrifying sludge. The microbial diversity of the R1 reactor was much greater than that of R2 and R3, indicating that a larger proportion of denitrifying bacteria were present in the R2 and R3 reactors. In addition, the batch experiments showed that the higher the initial pH, the higher the nitrite accumulation rate was.
将部分反硝化（NO -N→NO -N）与厌氧氨氧化（ANAMMOX）相结合，以实现低C/N比和低能耗的氮去除。研究了三种不同的驯化条件，即R1（缺氧条件下的序批式反应器（SBR））、R2（交替缺氧/好氧条件下的SBR）和R3（低强度曝气条件下的SBR），使用葡萄糖作为电子供体，在120天的运行过程中实现亚硝酸盐的持续积累。随后，研究了污泥的反硝化性能和微生物群落结构。结果表明，由于曝气导致溶解氧的存在，R2和R3反应器中驯化后的污泥比R1中的污泥具有更好的部分反硝化性能。值得注意的是，R3反应器具有亚硝酸盐积累的最佳条件。高通量测序分析表明，R2和R3中的优势细菌分别为 ，相对丰度分别为45.44%和34.96%。这是首次报道 作为反硝化污泥中的优势细菌。R1反应器的微生物多样性远大于R2和R3，表明R2和R3反应器中存在更大比例的反硝化细菌。此外，批次实验表明，初始pH值越高，亚硝酸盐积累速率越高。
