Département de Phytologie, F. S. A. A., Université Laval, Ste Foy, Québec, Canada G1K 7P4.
Appl Environ Microbiol. 1994 Feb;60(2):489-95. doi: 10.1128/aem.60.2.489-495.1994.
The in vitro production of chitinases and beta-1,3-glucanases by Stachybotrys elegans, a mycoparasite of Rhizoctonia solani, was examined under various culture conditions, such as carbon and nitrogen sources, pH, and incubation period. Production of both enzymes was influenced by the carbon source incorporated into the medium and was stimulated by acidic pH and NaNO(3). The activity of both enzymes was very low in culture filtrates from cells grown on glucose and sucrose compared with that detected on chitin (for chitinases) and cell wall fragments (for beta-1,3-glucanases). Protein electrophoresis revealed that, depending on the carbon source used, different isoforms of chitinases and beta-1,3-glucanases were detected. S. elegans culture filtrates, possessing beta-1,3-glucanase and chitinase activities, were capable of degrading R. solani mycelium.
我们研究了菌寄生真菌立枯丝核菌的拟青霉在不同培养条件下（如碳源和氮源、pH 值和培养时间）产生几丁质酶和β-1,3-葡聚糖酶的情况。这两种酶的产生均受到培养基中碳源的影响，并受到酸性 pH 值和 NaNO3 的刺激。与在几丁质（用于几丁质酶）和细胞壁片段（用于β-1,3-葡聚糖酶）上检测到的活性相比，在以葡萄糖和蔗糖为碳源的细胞培养滤液中，这两种酶的活性都非常低。蛋白质电泳表明，根据所用的碳源，检测到不同的几丁质酶和β-1,3-葡聚糖酶同工酶。具有β-1,3-葡聚糖酶和几丁质酶活性的拟青霉培养液能够降解立枯丝核菌菌丝体。
