Institute of Bacteriology and Animal Hygiene, University of Veterinary Medicine, A-1030 Vienna, Austria.
Appl Environ Microbiol. 1994 Jun;60(6):2155-7. doi: 10.1128/aem.60.6.2155-2157.1994.
Serum and/or glucose added to Actinobacillus pleuropneumoniae suspensions before freeze-drying significantly increased survival rates of bacteria in aerosols. Aerosols with predictable numbers of viable bacteria can be made as required in an aerosol infection model. Sucrose supplementation of impinger fluids increased recovery of viable A. pleuropneumoniae.
在冻干前向胸膜肺炎放线杆菌悬浮液中添加血清和/或葡萄糖会显著提高细菌在气溶胶中的存活率。可以根据需要在气溶胶感染模型中制备具有可预测数量的活菌的气溶胶。在冲击器液中添加蔗糖可提高胸膜肺炎放线杆菌的活菌回收率。