Jacobsen M J, Nielsen J P, Nielsen R
Danish Veterinary Laboratory, Copenhagen, Denmark.
Vet Microbiol. 1996 Apr;49(3-4):159-68. doi: 10.1016/0378-1135(95)00184-0.
An aerosol infection model for inoculation of pigs with Actinobacillus pleuropneumoniae is described. With this model the virulence of three A. pleuropneumoniae biotype 1 strains representing serotypes 2, 5b and 6, and one Danish biotype 2 were compared using 13-week-old pigs for inoculation. The pigs were sacrificed 24 h after aerosol exposure and lung lesions were evaluated. In pigs exposed to aerosols of suspensions containing 10(4) CFU/ml of serotypes 2, 5b and 6, a number of 5-10 lesions of haemorrhagic necrotizing pneumonia were induced. For the biotype 2 strain the dose creating similar lesions was 10(9) CFU/ml. Repeated experiments confirmed these results showing similar virulence of serotypes 2, 5b and 6 whereas the biotype 2 strain proved less virulent. The aerosol infection model allowed a comparison of the number of A. pleuropneumoniae CFU/liter air which were necessary to induce lung lesions in susceptible pigs. This indicates that the model will be well suited for virulence studies of A. pleuropneumoniae serotypes in pigs.
描述了一种用胸膜肺炎放线杆菌接种猪的气溶胶感染模型。利用该模型，使用13周龄的猪进行接种，比较了代表血清型2、5b和6的三种胸膜肺炎放线杆菌生物型1菌株以及一种丹麦生物型2菌株的毒力。在气溶胶暴露24小时后处死猪，并评估肺部病变。在暴露于含有10⁴CFU/ml血清型2、5b和6的悬浮液气溶胶的猪中，诱发了5-10处出血性坏死性肺炎病变。对于生物型2菌株，产生类似病变的剂量为10⁹CFU/ml。重复实验证实了这些结果，表明血清型2、5b和6具有相似的毒力，而生物型2菌株的毒力较低。该气溶胶感染模型能够比较在易感猪中诱发肺部病变所需的每升空气中胸膜肺炎放线杆菌CFU数量。这表明该模型非常适合用于猪胸膜肺炎放线杆菌血清型的毒力研究。
