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随机突变和定点突变对肌氨酸氧化酶底物特异性和最适 pH 值的改变。

Alteration of Substrate Specificity and Optimum pH of Sarcosine Oxidase by Random and Site-Directed Mutagenesis.

机构信息

Tsuruga Institute of Biotechnology, Toyobo Co., Ltd., Tsuruga, Fukui Prefecture 914, Japan.

出版信息

Appl Environ Microbiol. 1994 Nov;60(11):4213-5. doi: 10.1128/aem.60.11.4213-4215.1994.

DOI:10.1128/aem.60.11.4213-4215.1994
PMID:16349451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC201966/
Abstract

The sarcosine oxidase gene was randomly mutated, and F103V, which showed altered specificity, was screened with the plate assay. Five different mutants at position 103 were also constructed. Each mutant exhibited a rather striking change in substrate specificity, except for F103W. The optimum pH was also shifted, depending on the substrate.

摘要

该天冬氨酸氧化酶基因被随机突变，并用平板检测筛选出了具有改变的特异性的 F103V。在第 103 位还构建了另外 5 个不同的突变体。除了 F103W 外，每个突变体的底物特异性都发生了相当显著的变化。最适 pH 值也根据底物而改变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de47/201966/efad04e34f36/aem00028-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de47/201966/efad04e34f36/aem00028-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de47/201966/efad04e34f36/aem00028-0324-a.jpg

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本文引用的文献

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Purification and some properties of sarcosine oxidase from Corynebacterium sp. U-96.
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A fluorophotometric determination of serum creatinine and creatine using a creatinineamidohydrolase-creatineamidinohydrolase-sarcosine oxidase-peroxidase system and diacetyldichlorofluorescin.
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Bacterial sarcosine oxidase: comparison of two multisubunit enzymes containing both covalent and noncovalent flavin.细菌肌氨酸氧化酶：两种含有共价和非共价黄素的多亚基酶的比较
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Enzyme engineering for nonaqueous solvents: random mutagenesis to enhance activity of subtilisin E in polar organic media.非水溶剂中的酶工程：通过随机诱变提高枯草杆菌蛋白酶E在极性有机介质中的活性
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