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使用链终止抑制剂的DNA测序。

DNA sequencing with chain-terminating inhibitors.

作者信息

Sanger F, Nicklen S, Coulson A R

出版信息

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.

DOI:10.1073/pnas.74.12.5463
PMID:271968
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431765/
Abstract

A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.

摘要

本文描述了一种测定DNA核苷酸序列的新方法。它类似于“加减法”[桑格，F. & 库尔森，A. R.（1975年）《分子生物学杂志》94卷，441 - 448页]，但利用了正常脱氧核苷三磷酸的2'，3'-双脱氧和阿拉伯核苷类似物，这些类似物可作为DNA聚合酶的特异性链终止抑制剂。该技术已应用于噬菌体φX174的DNA，比加减法都更快速、更准确。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f14/431765/28fda87692be/pnas00043-0273-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f14/431765/c9aecf33ea8f/pnas00043-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f14/431765/339a7ca0dcd8/pnas00043-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f14/431765/28fda87692be/pnas00043-0273-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f14/431765/c9aecf33ea8f/pnas00043-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f14/431765/339a7ca0dcd8/pnas00043-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f14/431765/28fda87692be/pnas00043-0273-b.jpg

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DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.
2
2',3'-Dideoxy-3' aminonucleoside 5'-triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases.2',3'-二脱氧-3'-氨基核苷5'-三磷酸是DNA聚合酶催化的DNA合成的终止剂。
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2
Purine nucleosides. XII. The preparation of 2',3'-dideoxyadenosine, 2',5'-dideoxyadenosine, and 2',3',5'-trideoxyadenosine from 2'-deoxyadenosine.嘌呤核苷。十二。由2'-脱氧腺苷制备2',3'-二脱氧腺苷、2',5'-二脱氧腺苷和2',3',5'-三脱氧腺苷。
Biochemistry. 1966 Jan;5(1):224-31. doi: 10.1021/bi00865a029.
3
Purine nucleosides. XIV. Unsaturated furanosyl adenine nucleosides prepared via base-catalyzed elimination reactions of 2'-deoxyadenosine derivatives.
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