Centre for Plant Biotechnology, Department of Botany, University of Toronto, Erindale Campus, Mississauga, Ontario L5L 1C6, Canada.
Appl Environ Microbiol. 1994 Dec;60(12):4456-60. doi: 10.1128/aem.60.12.4456-4460.1994.
A uniparental mitochondrial (mt) transmission pattern has been previously observed in laboratory matings of the cultivated mushroom Agaricus bisporus on petri dishes. In this study, four sets of specific matings were further examined by taking mycelial plugs from the confluent zone of mated homokaryons and inoculating these plugs into rye grain for laboratory fruiting and for fruiting under industrial conditions. Examination of the mt genotype of each individual fruit body for mt-specific restriction fragment length polymorphisms further confirmed that the mt genome was inherited uniparentally. The vegetative radial growth and the fruiting activity of two pairs of intraspecific heterokaryons, each pair carrying the same combination of nuclear genomes but different mt genotypes, were compared. Our results suggested that the mt genotype did not appreciably affect radial growth or fruiting activity. The failure to recover both heterokaryons, each carrying either parental mt genotype in any given cross, therefore clearly indicated that in matings of A. bisporus, the mt genome from one of the parental homokaryons is either selectively excluded in the newly formed heterokaryon or selectively eliminated in the immediate heterokaryotic mitotic progeny of the newly formed heterokaryon.
曾在培养蘑菇 Agaricus bisporus 的实验室交配中观察到单亲线粒体（mt）传递模式。在这项研究中，通过从交配同核体的融合区取菌丝塞并将这些塞接种到黑麦粒中进行实验室结实和工业条件下结实，进一步检查了四组特定的交配。对每个个体果实体的 mt 基因型进行 mt 特异性限制性片段长度多态性的检查进一步证实了 mt 基因组是单亲遗传的。比较了两对种内异核体的营养径向生长和结实活性，每对携带相同的核基因组组合但具有不同的 mt 基因型。我们的结果表明，mt 基因型不会显著影响径向生长或结实活性。在任何给定的杂交中，都未能回收携带任一亲本 mt 基因型的两个异核体，这清楚地表明，在 A. bisporus 的交配中，来自一个亲本同核体的 mt 基因组要么在新形成的异核体中被选择性排除，要么在新形成的异核体的立即异核有丝分裂后代中被选择性消除。
