Centre for Plant Biotechnology, Department of Botany, University of Toronto, Erindale Campus, Mississauga, Ontario, Canada L5L 1C6, and Mushroom Experimental Station, Horst, The Netherlands.
Appl Environ Microbiol. 1992 Nov;58(11):3553-60. doi: 10.1128/aem.58.11.3553-3560.1992.
Ten heterokaryons of Agaricus bisporus (= Agaricus brunnescens) were shown to carry four different mitochondrial (mt) genotypes by analysis of mt restriction fragment length polymorphisms (RFLPs). Fifteen homokaryons derived from these strains were used to investigate mt inheritance in A. bisporus. One hundred eighty-nine pairings were performed in 25 different combinations. Pairings in 15 different combinations produced heterokaryons on the basis of nuclear RFLP analyses and/or fruiting trials. The mt genotype of each new intraspecies hybrid was examined by using mt RFLPs as genetic markers. Our results suggest the following. (i) Recombination between the mt genomes was not a common event. (ii) From most individual pairings, all heterokaryons carried the same mt genotype. (iii) Heterokaryons carrying either of the two possible mt genotypes were observed in certain crosses after modification of the pairing procedure. A biparental transmission pattern was demonstrated for some crosses, but there appears to be a preference for one of the mt genotypes to predominate in any specific pairing.
10 个双孢蘑菇（= 棕色蘑菇）异核体通过线粒体（mt）限制片段长度多态性（RFLP）分析显示携带四种不同的 mt 基因型。从这些菌株中获得的 15 个同核体用于研究双孢蘑菇中的 mt 遗传。在 25 种不同组合中进行了 189 次配对。基于核 RFLP 分析和/或结实试验，在 15 种不同组合中的配对产生了异核体。使用 mt RFLP 作为遗传标记检查每个新种内杂种的 mt 基因型。我们的结果表明：（i）mt 基因组之间的重组不是常见事件。（ii）大多数个体配对中，所有异核体携带相同的 mt 基因型。（iii）在修改配对程序后，在某些杂交中观察到携带两种可能的 mt 基因型之一的异核体。某些杂交显示出双亲传递模式，但在任何特定的配对中，似乎有一种 mt 基因型占优势的偏好。
