Bobst A M, Sinha T K, Pan Y E
Science. 1975 Apr 11;188(4184):153-5. doi: 10.1126/science.163495.
Electron spin resonance is used to detect RNA's that contain polyadenylate tracts. The method depends on the ability of RNA's that contain polyadenylate sequences to associate with poly(2'-deoxy-2'-fluoro)uridylic acid, which has been spin-labeled with 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidinooxyl. The formation of the hybridization product can be detected by monitoring the decrease in mobility of the spin probe.
电子自旋共振用于检测含有聚腺苷酸序列的RNA。该方法依赖于含有聚腺苷酸序列的RNA与已用4-(2-碘乙酰胺基)-2,2,6,6-四甲基哌啶氮氧化物进行自旋标记的聚（2'-脱氧-2'-氟）尿苷酸结合的能力。通过监测自旋探针迁移率的降低可以检测杂交产物的形成。
