尿苷酸丰富的寡核苷酸区域在HeLa细胞异质性核RNA中的出现。
Occurrence of uridylate-rich oligonucleotide regions in heterogeneous nuclear RNA of HeLa cells.
作者信息
Molloy G R, Thomas W L, Darnell J E
出版信息
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3684-8. doi: 10.1073/pnas.69.12.3684.
Abstract
Heterogeneous nuclear RNA molecules from HeLa cells contain a specific segment of about 30 nucleotides length that is largely (about 80%) uridylic acid. This oligo(U) segment is located predominantly in the larger (70S-90S) heterogeneous nuclear RNA molecules, and is essentially absent in messenger RNA and 45S ribosomal precursor RNA molecules. The oligo(U) hybridizes rapidly to cellular DNA, suggesting that it is transcribed from the repeated regions of the DNA.
摘要
来自海拉细胞的不均一核RNA分子包含一段长度约为30个核苷酸的特定片段，该片段大部分（约80%）为尿苷酸。这段寡聚（U）片段主要位于较大的（70S - 90S）不均一核RNA分子中，而在信使RNA和45S核糖体前体RNA分子中基本不存在。该寡聚（U）片段能迅速与细胞DNA杂交，这表明它是从DNA的重复区域转录而来的。
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