Varakina N N, Zeleneva I V, Polikarpochkina R T, Khavkin E E
Sov J Dev Biol. 1975 Jan;5(1):52-9.
The content of cytochromes a,b and c, the activity of marker enzymes of the matrix and inner membrane of the mitochondria: glutamate dehydrogenase and cytochrome oxidase, as well as the rate of absorption of O2 by root segments in the presence of respiratory substrates, oxygen, inhibitors of respiration, and dinitrophenol, were determined. The intensification of cell respiration in the phase of elongation is determined not so much by new formation of cytochrome components of the respiratory cycle (during this period there is an accumulation only of cytochrome c) as by reorganization of the respiratory cycle (primarily its portion NADH - cytochrome b) and synthesis of enzymes of the matrix.
测定了细胞色素a、b和c的含量,线粒体基质和内膜的标志酶——谷氨酸脱氢酶和细胞色素氧化酶的活性,以及在有呼吸底物、氧气、呼吸抑制剂和二硝基苯酚存在的情况下根段对氧气的吸收速率。伸长阶段细胞呼吸的增强,与其说是由呼吸循环中细胞色素成分的新形成(在此期间仅积累细胞色素c)决定的,不如说是由呼吸循环的重组(主要是其NADH - 细胞色素b部分)和基质酶的合成决定的。