Maister H G, Rogovin S P, Stodola F H, Wickerham L J
Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Illinois.
Appl Microbiol. 1962 Sep;10(5):401-6. doi: 10.1128/am.10.5.401-406.1962.
Tetraacetylphytosphingosine (TAPS) formation by the F-60-10 mating type strain of the yeast Hansenula ciferrii, previously observed on agar plates, has been shown to take place in submerged cultures. The optimal conditions for TAPS formation, and the correlation of TAPS production and sugar utilization under aerobic conditions, were studied in 10-liter fermentors. For each gram of glucose consumed, 5 mg of TAPS were formed; for each gram of yeast solids produced, 15 mg of TAPS were synthesized. A 750-liter pilot-plant run yielded 175 g of crude TAPS, which were obtained by hexane extraction of centrifuged yeast cells.
先前在琼脂平板上观察到的，汉逊酵母（Hansenula ciferrii）F-60-10交配型菌株合成四乙酰植物鞘氨醇（TAPS）的现象，现已证明在深层培养中也会发生。在10升发酵罐中研究了TAPS形成的最佳条件，以及有氧条件下TAPS产量与糖利用之间的相关性。每消耗1克葡萄糖，可形成5毫克TAPS；每产生1克酵母固体，可合成15毫克TAPS。在750升中试工厂的运行中，通过己烷萃取离心后的酵母细胞，获得了175克粗TAPS。
Zotero 插件