西弗瑞汉逊酵母NRRL Y-1031 F-60-10的基因组序列草图
Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.
作者信息
Schneider Jessica, Andrea Heiko, Blom Jochen, Jaenicke Sebastian, Rückert Christian, Schorsch Christoph, Szczepanowski Rafael, Farwick Mike, Goesmann Alexander, Pühler Alfred, Schaffer Steffen, Tauch Andreas, Köhler Tim, Brinkrolf Karina
机构信息
Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany.
出版信息
Eukaryot Cell. 2012 Dec;11(12):1582-3. doi: 10.1128/EC.00258-12.
Abstract
Wickerhamomyces ciferrii is a microorganism characterized by the production and secretion of large amounts of acetylated sphingoid bases, in particular tetraacetyl phytosphingosine. Here, we present the 15.90-Mbp draft genome sequence of W. ciferrii NRRL Y-1031 F-60-10 generated by pyrosequencing and de novo assembly. The draft genome sequence comprising 364 contigs in 150 scaffolds was annotated and covered 6,702 protein-coding sequences. This information will contribute to the metabolic engineering of this yeast to improve the yield and spectrum of acetylated sphingoid bases in biotechnological production.
摘要
西弗瑞威克汉姆酵母是一种能够大量产生和分泌乙酰化鞘氨醇碱，尤其是四乙酰植物鞘氨醇的微生物。在此，我们展示了通过焦磷酸测序和从头组装生成的西弗瑞威克汉姆酵母NRRL Y - 1031 F - 60 - 10的1590万碱基对草图基因组序列。该草图基因组序列由150个支架中的364个重叠群组成，经过注释，涵盖了6702个蛋白质编码序列。这些信息将有助于对该酵母进行代谢工程改造，以提高生物技术生产中乙酰化鞘氨醇碱的产量和种类。
相似文献
1
Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.
Eukaryot Cell. 2012 Dec;11(12):1582-3. doi: 10.1128/EC.00258-12.
2
Genome sequence of Wickerhamomyces anomalus DSM 6766 reveals genetic basis of biotechnologically important antimicrobial activities.
FEMS Yeast Res. 2012 May;12(3):382-6. doi: 10.1111/j.1567-1364.2012.00791.x. Epub 2012 Feb 23.
3
Knockout of the DNA ligase IV homolog gene in the sphingoid base producing yeast Pichia ciferrii significantly increases gene targeting efficiency.
Curr Genet. 2009 Aug;55(4):381-9. doi: 10.1007/s00294-009-0252-z. Epub 2009 May 26.
4
Metabolic engineering of the non-conventional yeast Pichia ciferrii for production of rare sphingoid bases.
Metab Eng. 2012 Jul;14(4):412-26. doi: 10.1016/j.ymben.2012.03.003. Epub 2012 Mar 18.
5
High-level production of tetraacetyl phytosphingosine (TAPS) by combined genetic engineering of sphingoid base biosynthesis and L-serine availability in the non-conventional yeast Pichia ciferrii.
Metab Eng. 2012 Mar;14(2):172-84. doi: 10.1016/j.ymben.2011.12.002. Epub 2011 Dec 10.
6
Biotechnological production of sphingoid bases and their applications.
Appl Microbiol Biotechnol. 2013 May;97(10):4301-8. doi: 10.1007/s00253-013-4878-x. Epub 2013 Apr 12.
7
Integrative transformation system for the metabolic engineering of the sphingoid base-producing yeast Pichia ciferrii.
Appl Environ Microbiol. 2003 Feb;69(2):812-9. doi: 10.1128/AEM.69.2.812-819.2003.
8
Production of tetraacetyl phytosphingosine (TAPS) in Wickerhamomyces ciferrii is catalyzed by acetyltransferases Sli1p and Atf2p.
Appl Microbiol Biotechnol. 2013 Oct;97(19):8537-46. doi: 10.1007/s00253-012-4670-3. Epub 2013 Jan 15.
9
[Fermentative production of tetraacetyl phytosphingosine: a review].
Sheng Wu Gong Cheng Xue Bao. 2023 Jun 25;39(6):2204-2214. doi: 10.13345/j.cjb.221021.
10
Combined application of targeted and untargeted proteomics identifies distinct metabolic alterations in the tetraacetylphytosphingosine (TAPS) producing yeast Wickerhamomyces ciferrii.
J Proteomics. 2013 Apr 26;82:274-87. doi: 10.1016/j.jprot.2013.03.002. Epub 2013 Mar 14.
引用本文的文献
1
A versatile genetic toolkit for engineering for tetraacetyl phytosphingosine production.
Front Bioeng Biotechnol. 2025 Apr 28;13:1586218. doi: 10.3389/fbioe.2025.1586218. eCollection 2025.
2
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides.
Synth Syst Biotechnol. 2024 Jul 31;10(1):1-9. doi: 10.1016/j.synbio.2024.07.005. eCollection 2025.
3
Differences in the Fatty Acid Profile, Morphology, and Tetraacetylphytosphingosine-Forming Capability Between Wild-Type and Mutant .
Front Bioeng Biotechnol. 2021 Jun 9;9:662979. doi: 10.3389/fbioe.2021.662979. eCollection 2021.
4
Expanding the Knowledge on the Skillful Yeast .
J Fungi (Basel). 2021 Jan 9;7(1):36. doi: 10.3390/jof7010036.
5
Redundans: an assembly pipeline for highly heterozygous genomes.
Nucleic Acids Res. 2016 Jul 8;44(12):e113. doi: 10.1093/nar/gkw294. Epub 2016 Apr 29.
6
Genomics and the making of yeast biodiversity.
Curr Opin Genet Dev. 2015 Dec;35:100-9. doi: 10.1016/j.gde.2015.10.008. Epub 2015 Nov 30.
7
Clade- and species-specific features of genome evolution in the Saccharomycetaceae.
FEMS Yeast Res. 2015 Aug;15(5):fov035. doi: 10.1093/femsyr/fov035. Epub 2015 Jun 10.
8
Genome Sequence of the Yeast Cyberlindnera fabianii (Hansenula fabianii).
Genome Announc. 2014 Aug 7;2(4):e00638-14. doi: 10.1128/genomeA.00638-14.
本文引用的文献
1
Metabolic engineering of the non-conventional yeast Pichia ciferrii for production of rare sphingoid bases.
Metab Eng. 2012 Jul;14(4):412-26. doi: 10.1016/j.ymben.2012.03.003. Epub 2012 Mar 18.
2
High-level production of tetraacetyl phytosphingosine (TAPS) by combined genetic engineering of sphingoid base biosynthesis and L-serine availability in the non-conventional yeast Pichia ciferrii.
Metab Eng. 2012 Mar;14(2):172-84. doi: 10.1016/j.ymben.2011.12.002. Epub 2011 Dec 10.
3
RAPYD--rapid annotation platform for yeast data.
J Biotechnol. 2011 Aug 20;155(1):118-26. doi: 10.1016/j.jbiotec.2010.10.076. Epub 2010 Oct 30.
4
Knockout of the DNA ligase IV homolog gene in the sphingoid base producing yeast Pichia ciferrii significantly increases gene targeting efficiency.
Curr Genet. 2009 Aug;55(4):381-9. doi: 10.1007/s00294-009-0252-z. Epub 2009 May 26.
5
The outer frontier: the importance of lipid metabolism in the skin.
J Lipid Res. 2009 Apr;50 Suppl(Suppl):S417-22. doi: 10.1194/jlr.R800039-JLR200. Epub 2008 Oct 31.
6
Novel sphingolipid derivatives promote keratinocyte differentiation.
Exp Dermatol. 2008 Dec;17(12):1004-16. doi: 10.1111/j.1600-0625.2008.00736.x.
7
Anti-microbial and -inflammatory activity and efficacy of phytosphingosine: an in vitro and in vivo study addressing acne vulgaris.
Int J Cosmet Sci. 2007 Jun;29(3):181-90. doi: 10.1111/j.1467-2494.2007.00378.x.
8
Epidermal sphingolipids: metabolism, function, and roles in skin disorders.
FEBS Lett. 2006 Oct 9;580(23):5456-66. doi: 10.1016/j.febslet.2006.08.039. Epub 2006 Sep 1.
9
Formation of Extracellular Sphingolipids by Microorganisms: IV. Pilot-Plant Production of Tetraacetylphytosphingosine by Hansenula ciferrii.
Appl Microbiol. 1962 Sep;10(5):401-6. doi: 10.1128/am.10.5.401-406.1962.
10
Genome evolution in yeasts.
Nature. 2004 Jul 1;430(6995):35-44. doi: 10.1038/nature02579.
Zotero 插件