Jacobson H, Jacobson L S
Department of Chemistry, Brooklyn College of the City University of New York, Brooklyn, New York.
Appl Microbiol. 1966 Nov;14(6):940-52. doi: 10.1128/am.14.6.940-952.1966.
Methods for continuous production of viruses, and operation of the virustat, an apparatus in which such production was accomplished, were studied. Continuous production requires a separate continuous host growth chamber, such as the chemostat, and a multiunit virus growth chamber into which the virus-inoculated host cells are led. Successful continuous output of MS-2 and varphiX174 viruses, the latter in lysates, over periods of several days and at titers approximating those of batch lysates, was observed. Design problems include chamber sizes and flow rates, growth of resistant mutants within both virus and host growth chambers, clogging by lysis debris, and the phenomenon of self-inoculation. The latter represents virus growth in the first section of the chamber in excess of the washout rate, leading to lack of need for virus inoculation after an initial period. Use of the virustat for production and research purposes will require some attention to the formation of resistant bacterial colonies at pockets and surface sites of limited washout. With the virustat as a continuous virus production device, continuous purification methods are desirable. Research use of the virustat in continuous mutagenic population studies would require suppression of self-inoculation by use of many sections in the chamber, and improved servo control of host populations at low concentrations.
研究了病毒连续生产的方法以及病毒稳定器（一种实现这种生产的装置）的操作。连续生产需要一个单独的连续宿主生长室，如恒化器，以及一个多单元病毒生长室，接种病毒的宿主细胞被引入该生长室。观察到MS - 2和φX174病毒在数天内成功连续产出，后者以裂解物形式产出，其滴度接近批量裂解物的滴度。设计问题包括腔室大小和流速、病毒和宿主生长室内抗性突变体的生长、裂解碎片造成的堵塞以及自我接种现象。后者是指腔室第一部分中的病毒生长超过洗脱速率，导致在初始阶段之后无需病毒接种。将病毒稳定器用于生产和研究目的时，需要注意在洗脱受限的口袋和表面部位形成抗性细菌菌落。将病毒稳定器用作连续病毒生产装置时，需要连续纯化方法。在连续诱变群体研究中对病毒稳定器的研究使用将需要通过在腔室中使用多个部分来抑制自我接种，并在低浓度下改进宿主群体的伺服控制。
