一株链球菌在分批培养中产生透明质酸。
Production of hyaluronic Acid by a streptococcal strain in batch culture.
作者信息
Holmström B, Ricica J
机构信息
Department of Bacteriology, Karolinska Institutet, Stockholm, Sweden.
出版信息
Appl Microbiol. 1967 Nov;15(6):1409-13. doi: 10.1128/am.15.6.1409-1413.1967.
Abstract
A simple method for the production and preparation of hyaluronic acid (HA) is described. Three media were tested, which all supported relatively good yields of cells, but only in one of them were appreciable amounts of HA formed. In this medium, HA was produced during the logarithmic growth phase and showed a molecular weight of 7.3 x 10. HA prepared from 24-hr cultures of the same organism had a molecular weight of only 2.6 x 10. The kinetics of HA production in batch culture were studied, as well as of cell production and glucose consumption.
摘要
本文描述了一种生产和制备透明质酸（HA）的简单方法。测试了三种培养基，它们都能支持细胞获得相对较高的产量，但只有其中一种培养基能形成可观量的HA。在这种培养基中，HA在对数生长期产生，分子量为7.3×10。从同一生物体的24小时培养物中制备的HA分子量仅为2.6×10。研究了分批培养中HA的生产动力学，以及细胞生产和葡萄糖消耗的动力学。
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J Bacteriol. 1964 Jun;87(6):1452-6. doi: 10.1128/jb.87.6.1452-1456.1964.
2
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J Biol Chem. 1964 Mar;239:726-8.
3
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Proc Soc Exp Biol Med. 1959 Oct;102:125-7. doi: 10.3181/00379727-102-25165.
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