Murphy J R, Frankenfeld J, Calvert J, Tilton R C
University of Connecticut Health Center, Hartford, Connecticut 06105.
Appl Microbiol. 1972 Aug;24(2):283-5. doi: 10.1128/am.24.2.283-285.1972.
Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.
在含有硫酸甲酯吩嗪的情况下，通过用二氯靛酚染色，在反应混合物中孵育后，在聚丙烯酰胺凝胶中证明了来自硫杆菌属菌株IV-85粗提取物的硫氰酸酶活性。发现染色系统的灵敏度为8×10摩尔亚硫酸盐。
