硫代硫酸盐硫转移酶（硫氰酸酶）活性的组织化学检测。

Histochemical detection of thiosulphate sulphurtransferase (rhodanese) activity.

作者信息

Tanka D, Gátai K

出版信息

Histochemistry. 1983;77(2):285-8. doi: 10.1007/BF00506572.

DOI:10.1007/BF00506572

Abstract

A histochemical method is described by which the activity of thiosulphate sulphurtransferase (E.C. 2.8.1.1., rhodanese) can be detected. The method gives an easy and quick determination of enzyme activity. The basis of the detection is the reducing effect of sulphite (SO 2/3) ions resulting from the enzyme reaction. Nitro-blue tetrazolium chloride can be used as an indicator and N-methylphenazonium-methosulphate as an accelerator for increasing electron transport. In mammals rhodanese is localized in the mitochondria.

摘要

本文描述了一种组织化学方法，通过该方法可检测硫代硫酸盐硫转移酶（E.C. 2.8.1.1.，硫氰酸酶）的活性。该方法能简便快速地测定酶活性。检测的基础是酶反应产生的亚硫酸根离子（SO₂/₃）的还原作用。氯化硝基四氮唑蓝可用作指示剂，N-甲基吩嗪硫酸甲酯用作增强电子传递的促进剂。在哺乳动物中，硫氰酸酶定位于线粒体。

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本文引用的文献

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