Darras F S, Lee C, Huprikar S, Rademaker A W, Grayhack J T
Department of Urology, Northwestern University Medical School, Chicago, Illinois 60611.
J Urol. 1992 Aug;148(2 Pt 1):432-40. doi: 10.1016/s0022-5347(17)36623-5.
A widely held view is that the role of testis in prostatic growth is through its ability to secrete androgen. Our earlier observation suggested a non-androgenic role for the testis, and perhaps the epididymis, in promoting growth of the ventral prostate in rats. The present study was conducted to evaluate the separate role of the testis and the epididymis in this phenomenon. In the first study, increasing quantities of silastic tubing filled with crystalline testosterone were implanted into adult Sprague-Dawley rats at the time of bilateral epididymo-orchiectomy or sham-operation. Twenty-eight days later, growth of the ventral prostate, as determined by fresh weight, DNA, and protein content, was significantly greater in sham-operated rats than in those receiving combined epididymo-orchiectomy, confirming our previous observation using dihydrotestosterone. In the second and third studies, rats were subjected to selective surgical procedures to evaluate the independent role of the testis and the epididymis. At the same time, 12 cm silastic tubing filled with testosterone or dihydrotestosterone were implanted subcutaneously into each of these animals for 28 days. Results indicated that the ventral prostate was significantly smaller in rats receiving the combined epididymo-orchiectomy than that of sham-operated controls. Simple orchiectomy or simple epididymectomy resulted in an increased weight of the ventral prostate between the two values obtained from the above two groups. Ligation of either the efferent duct or the vas deferens yielded ventral prostatic weights comparable to the androgen-treated, sham-operated controls. These results indicated that in order to achieve a maximal effect on androgen-supported growth of the ventral prostate, the presence of both the testis and the epididymis is required.
一种普遍的观点认为，睾丸在前列腺生长中的作用是通过其分泌雄激素的能力来实现的。我们早期的观察结果表明，睾丸，或许还有附睾，在促进大鼠腹侧前列腺生长方面具有非雄激素作用。本研究旨在评估睾丸和附睾在这一现象中的各自作用。在第一项研究中，在成年Sprague-Dawley大鼠进行双侧附睾-睾丸切除或假手术时，将填充有结晶睾酮的硅橡胶管数量逐渐增加地植入大鼠体内。28天后，通过鲜重、DNA和蛋白质含量测定发现，假手术大鼠的腹侧前列腺生长明显大于接受联合附睾-睾丸切除的大鼠，这证实了我们之前使用双氢睾酮的观察结果。在第二项和第三项研究中，对大鼠进行选择性手术操作，以评估睾丸和附睾的独立作用。同时，将填充有睾酮或双氢睾酮的12厘米硅橡胶管皮下植入每只动物体内28天。结果表明，接受联合附睾-睾丸切除的大鼠的腹侧前列腺明显小于假手术对照组。单纯睾丸切除或单纯附睾切除导致腹侧前列腺重量增加，介于上述两组获得的两个值之间。结扎输出小管或输精管后，腹侧前列腺重量与接受雄激素治疗的假手术对照组相当。这些结果表明，为了对雄激素支持的腹侧前列腺生长产生最大影响，睾丸和附睾都需要存在。
