Thorslund Sara, Sanchez Javier, Larsson Rolf, Nikolajeff Fredrik, Bergquist Jonas
Department of Engineering Sciences, Angström Laboratory, Uppsala University, Sweden.
Colloids Surf B Biointerfaces. 2005 Dec 30;46(4):240-7. doi: 10.1016/j.colsurfb.2005.10.009. Epub 2005 Dec 13.
A new composition of heparin coating for microfluidic systems made out of poly(dimethylsiloxane) (PDMS) was developed and evaluated. The coating that consists of a conditioning polyamine layer followed by two heparin/glutaraldehyde layers, resulted in channel surfaces with sufficient wettability to obtain flow of human normal plasma by capillary force alone. Hydrophilic channel walls are a desirable characteristic in microfluidic devices, since alternative pumping mechanisms must otherwise be included into the system. The immobilized heparin showed high antithrombin-binding capacity and a low degree of blood-material interaction. Plasma in contact with heparin-coated PDMS formed no detectable fibrin in a spectrophotometric assay by which plasma in contact with non-treated PDMS showed complete coagulation. The quartz crystal microbalance technique with energy dissipation monitoring (QCM-D) was utilized to obtain detailed information regarding adsorption kinetics and structural properties of the different layers composing the heparin coating.
一种用于由聚二甲基硅氧烷（PDMS）制成的微流控系统的新型肝素涂层组合物被研发并进行了评估。该涂层由一层预处理多胺层和两层肝素/戊二醛层组成，使通道表面具有足够的润湿性，仅靠毛细作用力就能实现人正常血浆的流动。亲水性通道壁是微流控装置中一个理想的特性，因为否则系统中必须包含其他泵送机制。固定化的肝素显示出高抗凝血酶结合能力和低程度的血液-材料相互作用。在分光光度测定中，与肝素涂层的PDMS接触的血浆未形成可检测到的纤维蛋白，而与未处理的PDMS接触的血浆则完全凝固。利用具有能量耗散监测的石英晶体微天平技术（QCM-D）来获取有关构成肝素涂层的不同层的吸附动力学和结构特性的详细信息。
