Xu Jun, Arnold Arthur P
Department of Physiological Science, UCLA, 621 Charles E. Young Drive South, Los Angeles, CA 90095-1606, USA.
Endocr Res. 2005;31(2):111-9. doi: 10.1080/07435800500229243.
Using Western blot analysis we found transcriptional co-repressor Sin3A to be expressed at a higher level in male mouse kidney than in females. HDAC1 (histone deacetylase 1) protein, another co-repressor forming complexes with Sin3A, was not higher in males. No sex differences in Sin3A expression were found after gonadectomy, suggesting that gonadal secretions in adulthood cause the sex difference in kidney expression of Sin3A. In contrast, HDAC1 levels were higher in castrated gonadal males than in females, which presumably reflects a long-lasting differentiating effect of testicular secretions in early development on this protein in kidneys. In gonadectomized mice in which sex chromosome complement (XX vs. XY) is independent of gonadal type (testes vs. ovaries), there was no difference in the level of Sin3A or HDAC1 expression in kidney in XX or XY mice of the same gonadal sex.
通过蛋白质印迹分析，我们发现转录共抑制因子Sin3A在雄性小鼠肾脏中的表达水平高于雌性。另一种与Sin3A形成复合物的共抑制因子HDAC1（组蛋白去乙酰化酶1）蛋白在雄性中并不更高。去势后未发现Sin3A表达的性别差异，这表明成年期性腺分泌导致肾脏中Sin3A表达的性别差异。相比之下，去势的性腺雄性中HDAC1水平高于雌性，这可能反映了早期发育中睾丸分泌对肾脏中该蛋白的长期分化作用。在性染色体组成（XX与XY）与性腺类型（睾丸与卵巢）无关的去势小鼠中，相同性腺性别的XX或XY小鼠肾脏中Sin3A或HDAC1的表达水平没有差异。
