反向遗传学系统在确定丝状病毒致病性中的作用。

The role of reverse genetics systems in determining filovirus pathogenicity.

作者信息

Theriault S, Groseth A, Artsob H, Feldmann H

机构信息

National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.

出版信息

Arch Virol Suppl. 2005(19):157-77. doi: 10.1007/3-211-29981-5_13.

DOI:10.1007/3-211-29981-5_13

PMID:16355872

Abstract

The family Filoviridae is comprised of two genera: Marburgvirus and Ebolavirus. To date minigenome systems have been developed for two Ebola viruses (Reston ebolavirus and Zaire ebolavirus [ZEBOV]) as well as for Lake Victoria marburgvirus, the sole member of the Marburgvirus genus. The use of these minigenome systems has helped characterize functions for many viral proteins in both genera and have provided valuable insight towards the development of an infectious clone system in the case of ZEBOV. The recent development of two such infectious clone systems for ZEBOV now allow effective strategies for experimental mutagenesis to study the biology and pathogenesis of one of the most lethal human pathogens.

摘要

丝状病毒科由两个属组成

马尔堡病毒属和埃博拉病毒属。迄今为止，已经为两种埃博拉病毒（雷斯顿埃博拉病毒和扎伊尔埃博拉病毒[ZEBOV]）以及马尔堡病毒属的唯一成员维多利亚湖马尔堡病毒开发了微型基因组系统。这些微型基因组系统的使用有助于表征这两个属中许多病毒蛋白的功能，并为开发ZEBOV的感染性克隆系统提供了有价值的见解。最近为ZEBOV开发的两种这样的感染性克隆系统，现在为实验诱变提供了有效的策略，以研究这种最致命的人类病原体之一的生物学和发病机制。